|
KMID : 1140120100150010034
|
|
Cancer Prevention Research
2010 Volume.15 No. 1 p.34 ~ p.38
|
|
|
Ethanol Upregulates PTEN via Nuclear Factor-kappa B (NF-κB) Signaling Pathways in HL-60 Cells
|
|
Jeong Young-Ju
Kim Jong-Suk
|
|
|
Abstract
|
|
|
The phosphatase tensin homolog deleted on chromosome 10 (PTEN) has various cellular functions including cell growth, proliferation, migration and cell survival. PTEN also plays an important role in ethanol-induced cytotoxicity. The present study was performed to determine the role of ethanol in PTEN expression of HL-60 cells. Here we show that ethanol up-regulates PTEN via activation of nuclear factor-kappa-light-chain-enhancer of activated B (NF-κB) in HL-60 cells. Western blot and RT-PCR results revealed that ethanol increased PTEN expression at HL-60 cells in a dose-dependent manner. In
addition, we found that ethanol activated the NF-κB pathways, evidenced by NF-κB DNA binding activity in ethanol-treated cells. However, ethanol-induced PTEN expression was completely blocked by disruption of NF-κB with pyrrolidine dithiocarbamate (PDTC). We conclude that ethanol induces up-regulation of PTEN expression through NF-κB activation in HL-60 cells.
|
|
|
KEYWORD
|
|
|
PTEN, NF-κB, Ethanol, HL-60 cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|